Saliva was collected prospectively from patients presenting with suspected dengue infection 4 to 8 days after the onset of symptoms and assayed by a commercial dengue immunoglobulin m igm and igg capture enzymelinked immunosorbent assay elisa panbio dengue duo elisa. Evaluation of the mrl diagnostics dengue fever virus igm capture elisa and the panbio rapid immunochromatographic. The test improvement resulted in a highly sensitive and specific test suitable for use as a firstline test in the field. We evaluated two new commercial dengue diagnostic tests, the mrl diagnostics dengue fever virus igm capture elisa and the panbio rapid immunochromatographic test, on serum samples collected during a dengue epidemic in jamaica. The clinical picture and the pathogenesis of the severe disease are explained. The mrl elisa method correctly identified 98% 78 of 80 of the samples as dengue positive, while the panbio test identified 100% 80 of 80. Evaluation of commercially available antidengue virus immunoglobulin m tests iii dengue igm capture elisa pb igmcap panbio diagnostics, australia18 pathozymedengue m pathozyme m omega diagnostics ltd. Antibodies to dengue if present in the specimen, will bind to the antihuman igg antibodies adsorbed onto the surface of the wells. In this study, two commercial tests for both igm and igg were compared panbio dengue duo igm and igg capture elisa and the venture technologies igm and igg dot blot. In order to assess combined diagnostic value of these tests, we study the kinetic profiles of circulating ns1, dengue igm and igg antibodies over the course of the disease by using an inhouse dengue type 1 denv1 specific ns1 capture elisa and the commercial panbio dengue igm and igg capture elisas. Enzymelinked immunoassay for dengue virus igm and igg. Sera from hospital attendees, with requests for denv antibody testing, were tested with the panbio dengue duo cassette and a reference antibody capture assay for the detection of igm dengue igm capture elisa national institute of virology, india and igg dengue igg capture. If both ns1 and igm positive results were combined, the detection rate increased to 89.
Comparison of panbio dengue duo igm and igg capture elisa. All convalescent samples were tested only for anti dengue igm antibodies. Dengue diagnostic test for the resource constraint. The test improvement resulted in a highly sensitive and speci. Laboratory diagnosis was based on virus isolation and on hemagglutination inhibition hai assay and an inhouse igm and. Diagnosis of dengue by igm capture elisa with principle. Commercial tests, namely, the panbio dengue igm capture elisa system panbio, sinnamon park, australia and pathozyme dengue m omega diagnostics, alva, uk, were performed according to the manufacturers protocols. Evaluating the sensitivity of a commercial antigen capture. Identification of the substancemixture and of the companyundertaking cat.
Comparison of two generations of the panbio dengue ns1 capture enzymelinked immunosorbent assay monique da rocha. This document describes the use of an igm antibody capture enzyme linked immunosorbent assay mac elisa for the. Panbio dengue igg indirect elisa virotech diagnostics. A commercially available enzymelinked immunosorbent assay elisa panbio dengue screening elisa that utilized both immunoglobulin m igm and igg capture in the same microtiter well for the diagnosis of dengue infection was evaluated. Dengue igg indirect antihuman igg hrp conjugate tmb chromogen wash buffer this material is stable under recommended storage and handling conditions. Enzymelinked immunosorbent assay elisa for immunoglobulin m igm. Dengue specific antibodies were tested by igm capture elisa and ict. Comparison of a dipstick dotelisa with commercial assays. None of the 43 confirmed non dengue cases and 20 healthy individual samples were positive by ns1 elisa giving specificity of 100%. Dengue igg microlisa test is an enzyme immunoassay based on gac capture elisa. Evaluation of a rapid immunochromatographic device for the. Video describing the steps undertaken in performing elisa to detect igm antibodies against dengue in patient serum. Anti dengue igm was measured using the dengue igm capture elisa kit panbio, brisbane, australia.
Panbio dengue igm capture elisa wash buffer, sample diluent, antigen diluent buffer, dengue 14 antigen, hrp conjugate 1. Evaluation of the mrl diagnostics dengue fever virus igm capture elisa and the panbio rapid immunochromatographic test for diagnosis of dengue fever in jamaica. Evaluation of commercially available anti dengue virus immunoglobulin m tests iii dengue igm capture elisa pb igmcap panbio diagnostics, australia18 pathozyme dengue m pathozyme m. Not for sale or distribution in the united states of america page 1 of 5 revised 112008 eden01meden01m05 en dengue igm capture elisa cat no. The performances of the mrl dengue fever virus immunoglobulin m igm capture enzymelinked immunosorbent assay elisa and the panbio dengue duo igm capture and igg capture elisa. Diagnosis of dengue by igm capture elisa with principle, procedure and result interpretation description of the test. Pdf the objectives of this study were to conduct a further evaluation of performance characteristics. Comparison of two commercial elisa kits for the detection. The use of ns1 rapid diagnostic test and qrtpcr to. Request pdf comparison of panbio dengue duo igm and igg capture elisa and venture technologies dengue igm and igg dot blot a number of commercial elisa for dengue diagnosis have recently. Meanwhile, a total of 58 samples were negatively tested for both dengue ns1 and dengue igm by panbio dengue early rapid test and panbio dengue igm capture elisa respectively. Citeseerx document details isaac councill, lee giles, pradeep teregowda.
Of these ns1 antigen alone was positive in 48, igg was positive in 24, igm was positive in 31 and both ns1, igm. Sera were tested for immunoglobulin g igg panbio dengue indirect igg elisahightiter capture and immunoglobulin m igm panbio antibodies. Testing with both sd bioline dengue duo rapid test and elisa was done after separating the serum from all. Citeseerx evaluation of the mrl diagnostics dengue fever. Dengue fever testing is used to determine whether a person with symptoms and recent potential exposure to dengue has been infected. Recommended test is ns1 antigen by elisa in the first 5 days of fever.
Not for sale or distribution in the united states of america. Out of the 228 samples tested, 157 were positive by mac elisa and 111 were found to be positive for dengue infection by rapid ict. Antihuman igg antibodies are coated onto microtiter wells. Pdf comparison of panbio dengue duo igm and igg capture. Dengue igm microlisa is designed for invitro qualitative detection of dengue igm antibodies in human serum or plasma and is used as a screening test for testing of collected blood samples suspected for dengue. Platelia dengue igg capture is an immunocapture assay designed to detect the specific high level of dengue igg. Dengue igm capture elisa assay working sheet samples to. Apr 02, 2010 video describing the steps undertaken in performing elisa to detect igm antibodies against dengue in patient serum. Dengue igg igm elisa kit parasitology antibody detection elisa kits. We evaluated the improvement of the ns1 antigen capture elisa dengue early elisa from panbio compared to results previously obtained with the. Evaluation of a commercial dengue combo rapid test kit for. Comparison of two commercial elisa kits for the detection of. Diagnosis was based on a hemagglutination inhibition hai assay, with patients having either primary dengue n 90, secondary. A number of commercial elisa for dengue diagnosis have recently become available, though direct comparison between these assays have not.
Recently, two commercial elisas that are based on these different strategies have been released. Eden01m eden01m05 intended use the panbio dengue igm capture elisa is for the qualitative detection of igm antibodies to dengue antigen in serum, as an aid. Clinical features and pitfalls in the laboratory diagnosis of. Enhanced performance of an innovative dengue iggigm rapid. Dengue diagnostic test for the resource constraint developing. Panbio dengue igm capture elisa virotech diagnostics. Sensitivity in primary and secondary dengue was 95%, while specificity was 94%. Performance of commercial dengue ns1 elisa and molecular. The venture technologies dengue igm and igg dot blot assays and the.
The endemicity of dengue virus denv infection remains a major public health problem in lao pdr. Pdf comparison of panbio dengue igm elisa assay with pentax. Dengue igg microlisa test is an enzyme immunoassay based on gaccapture elisa. The panbio dengue duo elisa gave the best distinction between dengue and other infections, as judged by. Comparison of panbio dengue duo enzymelinked immunosorbent.
Comparison of panbio dengue duo igm and igg capture elisa and venture technologies dengue igm and igg dot blot. We describe the virus characteristics and risk factors for dengue and dhf, and compare incidence and the case fatality rates in endemic regions southeast asia, western pacific, and the americas. Evaluation of the mrl diagnostics dengue fever virus igm. Comparison of panbio dengue igm elisa assay with pentax dengue igm particle agglutination assay. The main limitation of the study is that the rdt performance was not compared with other standard tests, such as ns1 or igm capture based elisa or rtpcr. Relevant identified uses of the substance or mixture and uses advised against. Comparison of a dipstick dotelisa with commercial assays for. Cross reactivity is seen in the flavivirus group between dengue virus, murray valley encephalitis. An acute primary infection with dengue virus can be identified by igm. The niv igm capture elisa mac elisa showed a high positivity rate 38. This study evaluated two newly introduced commercial tests for the detection of antibodies to dengue virus, the mrl diagnostics dengue fever virus igm capture elisa cypress, calif. Evaluation of immunoglobulin m and g capture enzymelinked.
Evaluation of the diagnostic accuracy of a new dengue iga. Relevant identified uses of the substance or mixture and uses advised against not available not available safety data sheet sds according to regulation ec no 4532010 panbio dengue igm capture elisa doc. Denv antibody testing, were tested with the panbio dengue. A total of 154 samples from individuals with febrile illness having dengue fever dflike symptoms were tested. Assay elisa and mrl dengue fever virus immunoglobulin m. Not for sale or distribution in the united states of. Assays for the serological diagnosis of dengue infections dengue igmcapture elisa panbio dengue iggindirect elisa panbio dengue ns1 antigen detection elisa panbio trainer. Platelia dengue igg capture is designed to detect ns1 specific igg useful to differentiate primary and secondary dengue virus infections 6, 7. Comparative analysis of the platelia dengue iga capture assay and the panbio dengue igm capture elisa. The drde dengue dipstick dot elisa was performed on the basis of the dot elisa principle. Fever virus igm capture elisa, pan bio dengue duo igm and igg. Evaluation of commercially available antidengue virus. Dengue igm capture elisa is an enzyme linked immunosorbent assay for the qualitative detection of igm antibodies against dengue virus antigen in human serum. Only serological assays of dengue specific igm by igm capture elisa were carried out on the convalescent.
The panbio dengue igm capture elisa is for the qualitative detection of igm antibodies to dengue antigen in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with dengue fever. The venture technologies dengue igm and igg dot blot assays and the panbio dengue duo igm and igg capture elisa were compared. Eighty sera from patients with dengue virus infections, 24 sera from patients with japanese encephalitis je, and 78. The panbio dengue screening elisa also detected 20 of 20 igmpositive. Card test brisbane, ustralia and the panbio microwell igm elisa with an igm capture elisaa national institute of virology, pune, india. The panbio dengue igm capture elisa is for the qualitative detection of igm antibodies to dengue antigen in serum, as an aid in the clinical. Dengue igm levels obtained by 2 different testing kits in 12 samples from 10 confirmed dengue imported cases in new caledonia, 20052006. Pdf evaluation of the mrl diagnostics dengue fever virus.
Comparison of panbio dengue duo igm and igg capture elisa and. Evaluation of a capture screening enzymelinked immunosorbent. Specimens and controls are added to the microtiter wells and incubated. The infection is difficult to diagnose without laboratory tests because symptoms may initially resemble other diseases, such as malaria. In practice, however, often only one blood sample is available from febrile travellers returning from dengue endemic areas. An antigen capture enzymelinked immunosorbent assay reveals high levels of the dengue virus protein nsi in the sera of. Jan 16, 2018 in this study undertaken between april and may 2015, a total of 277 adult participants were recruited from households across three localities in the sungai segamat subdistrict in segamat district. Comparison of seven commercial antigen and antibody enzyme. Eden01g intended use the panbio dengue igg indirect elisa is for the qualitative detection of igg antibodies to dengue antigen serotypes 1, 2, 3 and 4 in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms and past exposure consistent with dengue fever. Panbio dengue igg indirect elisa wash buffer, sample diluent, hrp conjugate 1.
Pdf the performances of the mrl dengue fever virus immunoglobulin m igm capture. Igg ratios in an igm capture elisa with acute and convalescentphase specimens taken at an interval of approximately 7 days. Eighty sera from patients with dengue virus infections, 24 sera from patients with japanese encephalitis je, and 78 sera from patients with nonflavivirus infections, such as malaria, typhoid. The dengue fever elisa test is a qualitative enzyme immunoassay for the detection of antibodies to dengue, in.
Comparison of two generations of the panbio dengue. Once the ns1 antigen igm igg antibody in patients serum was bound, conjugated monoclonal antibody was detected by adding the tetramethylbenzidine tmb substrate. Jan 19, 2011 we compared two generations of panbio brisbane, australia commercial kits for ns1 antigen capture for early diagnosis of dengue. This assay doesnt detect low level of dengue igg ab present from past infection. Seven commercial assays were evaluated to determine their suitability for the diagnosis of acute dengue infection. Jul 21, 2006 several enzymelinked immunosorbent assay elisa kits are commercially available for the rapid diagnosis of dengue infection, and have demonstrated good sensitivity and specificity in paired serum samples. The reproducibilty of dengue igm and igg elisa was studied in serum and filter paper blood spots from vietnamese febrile patients. A number of commercial elisa for dengue diagnosis have recently become available, though direct comparison between these assays have not been published. Evaluation of the mrl diagnostics dengue fever virus igm capture elisa and the panbio rapid immunochromatographic test for diagnosis of dengue fever in jamaica 1999 cached. Thus, the authors concluded that panbio igm based rdt alone would be a costeffective and sensitive option especially during the times of outbreak in dengue endemic settings. Test for detection of dengue ns1 antigen and denguespecific igm using 98 samples that were screened initially using panbio dengue igm capture elisa and panbio dengue.
The performances of the mrl dengue fever virus immunoglobulin m igm capture enzymelinked immunosorbent assay elisa and the panbio dengue duo igm capture and igg capture elisa were compared. The panbio dengue duo elisa gave the best distinction between dengue and other infections, as judged by the sum of sensitivity and specificity. Frequency and clinical manifestations of dengue in urban. In that study, the iga index assay displayed a sensitivity of 93% 1244. Details of the supplier of the safety data sheet manufacturersupplier.
Detection of dengue virus rna and serotype identification was performed using the procedure described by lanciotti et al. The panbio dengue igm capture elisa should be used in conjunction with other dengue serology. Detection of specific antibodies in saliva during dengue. Comparison of two generations of the panbio dengue ns1. Two commercial dengue diagnostic kits, panbio dengue igm capture and dengue igg capture elisa brisbane, australia were evaluated. A commercially available capture enzymelinked immunosorbent assay elisa for the detection of specific immunoglobulin m igm and igg antibodies produced during dengue infection panbio dengue duo was evaluated with paired serum specimens from 176 patients. Evaluation of rapid ict in comparison with macelisa in. Seroprevalence of dengue among healthy adults in a rural. The presence of antidenv igm and igg antibodies were detected using the sd dengue igm and igg capture elisa. We compared two generations of panbio brisbane, australia commercial kits for ns1 antigen capture for early diagnosis of dengue. Assessment of a rapid test with elisa done for the detection of.
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